Will my graphics card work in all PCIe slots?

What benefits will you have at PCIe gen 4 with a graphics card? Absolutely nothing, really Running PCIe or will not make a difference

A PCIe slot will not bottleneck the card But what CPU are you running? That might be a different story 6152021 EVGA_JacobF Nope

pcie 4 in pcie 3 slot ssd — The most common use for a PCIe x16 slot is a graphics card, or GPU Heavy PCIe Gen 3, requiring PCIe or newer: GPU, Required PCIe Gen & Lane

pciex16 slot configuration — The most common use for a PCIe x16 slot is a graphics card, or GPU Heavy PCIe Gen 3, requiring PCIe or newer: GPU, Required PCIe Gen & Lane